![]() ![]() ![]() However, for a complete understanding of evolutionary processes and other fundamental questions in biology, high-quality reference genome assemblies of all species are required. Draft genomes generated using more affordable second-generation sequencing technologies could be assembled for a larger number of species, but these were of lower quality because they were highly fragmented and annotation was erroneous. ![]() In the past, the generation of reference assemblies was prohibitively expensive and labour-intensive, so they were only produced for humans and the most important model organisms, and still contained gaps and errors. Reference genome assemblies provide a map of a species’ DNA sequence and its spatial context-that is, where along the chromosomes a specific piece of DNA sequence can be found.
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